Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Epidermis

Cell type

Foreskin

MeSH Description

The double-layered skin fold that covers the GLANS PENIS, the head of the penis.

Sample

source_name

Foreskin

cell type

foreskin, NHM2

passage

8

antibodies

H3K27ac (Abcam, ab177178)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Native ChIP was done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

61376379

Reads aligned (%)

98.9

Duplicates removed (%)

6.1

Number of peaks

7374 (qval < 1E-05)

hg19

Number of total reads

61376379

Reads aligned (%)

98.4

Duplicates removed (%)

6.7

Number of peaks

7170 (qval < 1E-05)