Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Epidermis

Cell type

501A

Primary Tissue

Skin

Tissue Diagnosis

Melanoma

Sample

source_name

Cell culture

cell line

501MEL

passage

N/A

antibodies

H3K4me1 (Abcam, ab8895)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Native ChIP was done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95). Libraries for ChIP-seq were done as previously described (Hasson D., et al., The octamer is the major form of CENP-A nucleosomes at human centromeres. Nat Struct Mol Biol. 2013 Jun;20(6):687-95).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

50170897

Reads aligned (%)

97.3

Duplicates removed (%)

10.9

Number of peaks

57530 (qval < 1E-05)

hg19

Number of total reads

50170897

Reads aligned (%)

97.0

Duplicates removed (%)

11.4

Number of peaks

56602 (qval < 1E-05)