Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SIRT1

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
ChipSeq reads from K562 wt early S phase
cell line
K562
cell type
Chronic myelogenous leukemia
chip antibody
anti-T530 phosphorylated SIRT1 antibody (ab156585, Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA from asynchronous cell cultures was isolated, size fractionated and subject to exunuclease digestion as described in Aladjem et al., Science 281, 1005, 1998. and Wang et al., Molecular and Cellular Biology 24:3373-86, 2004. DNA from asynchronous cell cultures was isolated, size fractionated and subject to exunuclease digestion as described in Aladjem et al., Science 281, 1005, 1998. and Wang et al., Molecular and Cellular Biology 24:3373-86, 2004. For ChipSeq, DNA molecules were enriched using each antibodies. Enriched DNA molecules were then amplified randam primer with klenow fragment and sequenced by Illumina Genome Analyzer IIx. Illumina Genomic DNA protocol (Illumina Paired-END DNA Sample Prep Kit).

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
17157322
Reads aligned (%)
38.4
Duplicates removed (%)
12.4
Number of peaks
8234 (qval < 1E-05)

hg38

Number of total reads
17157322
Reads aligned (%)
39.6
Duplicates removed (%)
12.1
Number of peaks
8271 (qval < 1E-05)

Base call quality data from DBCLS SRA