Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESCs_control shRNA_H3K9_ChIP-seq

strain background

12910la

cell line

J1

cell type

mouse embryonic stem cells (mESCs)

transduced with

lentiviral vector encoding for a control shRNA

chip antibody

H3K9me3 (Diagenode, pAb-056-050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was prepared from 10 million cells as previously described (Rowe et al., 2013b). Libraries were ligated with Illumina adaptors

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

30884353

Reads aligned (%)

96.2

Duplicates removed (%)

18.3

Number of peaks

487 (qval < 1E-05)

mm9

Number of total reads

30884353

Reads aligned (%)

96.0

Duplicates removed (%)

18.4

Number of peaks

470 (qval < 1E-05)