GSM2471000: T het H3K27me3 ChIPseq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
mESC derived mesodermal cells
NA
NA
Attributes by original data submitter
Sample
source_name
Early mesodermal enriched
strain background
129S2/C57Bl6J
genotype/variation
wild type control (T_het)
cell type
ES derived mesodermal enriched
chip antibody
H3K27me3 polyclonal antibody
chip antibody vendor
Diagenode
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. 20,00 T positive cells were FACS sorted directly into RLT RNA Lysis buffer (Qiagen) and RNA extarcted according to the manufactureres protocol. Libraries for ChIP-seq were constructed accoording to the Illumina TrueSeq ChIP Sample Preparation Kit. Libraries for RNA-seq were constructed according to the ScriptSeq v2 Complete (Mouse/Human/Rat) Low Input Kit (Illumina/Epicenter)