High-glucose Dulbecco's modified Eagle medium supplemented with 10% fetal calf serum, penicillin (100 U/mL), streptomycin (100mg/mL), 0.1mM L-Asparagine, and 50μM 2-mercaptoethanol
chip antibody
anti-MYC (ab32072, Abcam)
strain
C57BL/6
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate were isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated using relevant antibodies. For BRD4, RNA Polymerase II, IRF1, STAT1, H3K27Ac ChIP-Seq, indexed libraries were prepared using KAPA Hyper Prep Kit for Illumina platforms (Kapa Biosystems) and SeqCap Adapter Kit (Roche). For MYC ChIP-Seq, indexed libraries were prepared using Nugen Ovation Ultralow system (v2, Nugen protocol M01379v3).