Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H4K16ac
Cell type Class
Cell line
Cell type
Kc167

Cell type information


Source
e/se
Developmental Stage
dorsal closure stage

Attributes by Original Data Submitter


source_name
Kc cells
cell line
Kc
gender
female
treatment
GFP RNAi
chip antibody
anti-H4K16ac Active Motif (#39167)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments (Covaris) were performed according to (Schauer et al., 2013) adapted to cell lines (Villa et al., 2016). Briefly, cells were re-suspended in ice-cold homogenization buffer and fixed with 1% formaldehyde for 10 minutes at room temperature. After quenching with 125 mM glycine, the cells were collected, washed and sonicated in RIPA buffer with a Covaris sonifier (PIP: 140, DF 20%, CB: 200) for 30 min. MSL2 antibody was previously described in (Villa et al., 2012) and H4K16ac was acquired from Active Motif (#39167).

Platform Information


instrument_model
Illumina HiSeq 1500

External Database Query

Logs in read processing pipeline


Number of total reads
22861352
Reads aligned (%)
93.6
Duplicates removed (%)
10.5
Number of peaks
6768 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA