Antigen

Antigen Class

Histone

Antigen

H3.Y

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa (Kyoto), GFP-H3.Y ChIP

cell line

HeLa (Kyoto)

transfected plasmid

pIRESneo-EGFP

chip antibody

GFP (Abcam, ab290, lot GR278073-1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

HeLa Kyoto cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were subjected to chromatin shearing to an average size of 150 bp. Histone-DNA complexes were isolated with GFP-antibody. All libraries were prepared with the MicroPlex library preparation kit v2 (order no C05010012) following manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

52813562

Reads aligned (%)

98.9

Duplicates removed (%)

3.9

Number of peaks

35546 (qval < 1E-05)

hg19

Number of total reads

52813562

Reads aligned (%)

98.7

Duplicates removed (%)

4.3

Number of peaks

35401 (qval < 1E-05)