GSM2467347: GFP-H3.3 replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3.3
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa (Kyoto), GFP-H3.3 ChIP
cell line
HeLa (Kyoto)
transfected plasmid
pIRESneo-EGFP
chip antibody
GFP (Abcam, ab290, lot GR278073-1)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
HeLa Kyoto cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were subjected to chromatin shearing to an average size of 150 bp. Histone-DNA complexes were isolated with GFP-antibody. All libraries were prepared with the MicroPlex library preparation kit v2 (order no C05010012) following manufacturer's instructions.