GSM2466414: ATRA ATAC-seq rep2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
ATRA treated HL-60 cells
cell line
HL-60; ATCC CCL-240
tissue origin
peripheral blood
disease
acute promyelocytic leukemia
treated with
1μM ATRA for 4 days
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
samples were lysed in lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2 and NP-40) for 10 min on ice to prepare the nuclei. Immediately after lysis, nuclei were spun to remove the supernatant. Nuclei were then incubated with the Tn5 transposome and tagmentation buffer at 37 °C for 30 min (Vazyme, China). After the tagmentation, the stop buffer was added directly into the reaction to end the tagmentation. PCR was performed to amplify the library for 15 cycles. After the PCR reaction, libraries were purified with the 1.2× AMPure beads (Beckman, Germany). In house protocol which is similar as NEB library constructions (#7370)