GSM2442441: 5790 NPC Pol II-Ser5P ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Neural
Cell type
Neural progenitor cells
NA
NA
Attributes by original data submitter
Sample
source_name
Cortex
tissue
cortex
cell type
Neural progenitor cells
age
Embryonic day 15.5
strain
C57BL/6J-Tg(Dcx-mRFP)xC57BL/6J-Tg(Nestin-GFP)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Heterozygous Nestin-GFP mice were bred with homozygous DCX-mRFP mice to yield GFP/RFP double positive embryos. The cortices derived from the double positive embryos were dissociated by trituration in HBSS with 2mM of EDTA and 25 ug/ml of DNase I. Cells were washed once with HBSS and re-suspended in DMEM/F12. Fluorescence-activated cell sorting (FACS) was used to acquire neural progenitor cells (NPCs, green color) and neurons (double color). Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. DNA samples after ChIP were subjected to amplification with MicroPlex Library Preparation kit (AB-004-0012, Diagenode) according to the instruction.