Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Naive T cells

NA

NA

Sample

source_name

Naïve CD4+ T cells, activated with anti-CD3/CD28 for 24 hrs

strain

C57BL/6

tissue

spleen and lymph node

cell type

Th0

time point

24h

genotype

IRF1 KO

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells harvest was followed by a transposition reaction and purification (Buenrostro et al. Curr Protoc Mol Biol. 2015) Prepared as in Buenrostro et al. Curr Protoc Mol Biol. 2015

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

9687622

Reads aligned (%)

90.9

Duplicates removed (%)

34.7

Number of peaks

4599 (qval < 1E-05)

mm9

Number of total reads

9687622

Reads aligned (%)

90.8

Duplicates removed (%)

34.8

Number of peaks

4576 (qval < 1E-05)