Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
Naïve CD4+ T cells, activated with anti-CD3/CD28 for 72 hrs in the presence of IL27
strain
C57BL/6
tissue
spleen and lymph node
cell type
Tr1
time point
72h
genotype
IRF1 KO

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells harvest was followed by a transposition reaction and purification (Buenrostro et al. Curr Protoc Mol Biol. 2015) Prepared as in Buenrostro et al. Curr Protoc Mol Biol. 2015

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
28692935
Reads aligned (%)
91.0
Duplicates removed (%)
48.8
Number of peaks
17618 (qval < 1E-05)

mm9

Number of total reads
28692935
Reads aligned (%)
91.0
Duplicates removed (%)
48.8
Number of peaks
17598 (qval < 1E-05)

Base call quality data from DBCLS SRA