GSM2436543: ChIP-Seq analysis of H2BK120-ub in mouse ESCs under shControl knockdown treatment replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2BK120ub
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC shControl WITH LIF
cell type
mouse embryonic stem cells
chip antibody
H2Bk120-ub, clone 56 (Millipore, 05-1312)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million cells were treated withmicrococcal nuclease to generate mono-nucleosomes and for the ChIP chromatin was precipitated using 10 micrograms of antibody against H2Bk120-ub, clone 56 (Millipore, 05-1312). Libraries were generated as described before (Strikoudis A. et. al., 2016), including end-repair, A-tailing, adapter (Illumina Truseq system) ligation and PCR amplification.