Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Gonad
Cell type
Germline stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
GSCs
tissue
germline stem cells (GSCs)
genotype
Kmt2bF/F
chip antibody
H3K27me3 (Millipore, 07-449)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were crosslinked and chromatin was prepared according to a previous report (Nat. Protoc. 3, 1032–1045) with modifications. For RNA-seq, total RNA was purified using Isogen (Nippon Gene), and treated with DNaseI to digest genomic DNA. ChIP-seq and RNA-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), respectively, according to manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
36918740
Reads aligned (%)
95.6
Duplicates removed (%)
41.4
Number of peaks
1141 (qval < 1E-05)

mm9

Number of total reads
36918740
Reads aligned (%)
95.2
Duplicates removed (%)
41.4
Number of peaks
1172 (qval < 1E-05)

Base call quality data from DBCLS SRA