Epiblast stem cells cultured in Nodal inhibited medium
chip antibody
H3K27ac (Active motif,39133)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were collected and fixed in 1% formaldehyde solution and quenched by 0.125M glycine. Fixed cells were fragmented to a size range of 200-500bp by using Bioruptor Pico. Solubilized fragmented chromatin was immunoprecipitated with antibodies.Fragmented DNA was extracted with phenol-chloroform and precipitated with ethanol DNA fragments acquired from immunoprecipitation would be subjected to end-repaired, adaptor ligation and PCR amplification under the instruction of the manufacturers (New England Biolabs E7370 ).