ChIP Seq of Ser2P Pol II in Mago Knockdown condition
chip antibody
anti Ser2P (Abcam, catalog# Ab5095, lot# GR154006)
cell line
S2R+ Cells
cell type
Embryonic
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 _l of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA library preperation kit, following manufacturer's protocol. Custom adaptors with front tag were used in the protocol.