Antigen

Antigen Class

TFs and others

Antigen

POU5F1

Cell type

Cell type Class

Epidermis

Cell type

BJ

Primary Tissue

Skin

Tissue Diagnosis

Normal

Sample

source_name

OCT ChIP BJ

cell line

BJFOXA2 line

source cell tyep

BJ Fibroblast

genotype/variation

Clonal FOXA2 doxycycline inducible cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were generated as previously described (Mikkelsen et al., 2010, Gifford et al., 2013) and Libraries were sequenced on a HiSeq 2500 at 11pmol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

18427708

Reads aligned (%)

97.8

Duplicates removed (%)

4.4

Number of peaks

4930 (qval < 1E-05)

hg19

Number of total reads

18427708

Reads aligned (%)

97.2

Duplicates removed (%)

4.8

Number of peaks

4826 (qval < 1E-05)