GSM2430671: FOXA2 Mimosine Released ATAC Rep2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
FOXA2 Mimosine Released ChIP
cell line
BJFOXA2 line
source cell type
BJ Fibroblast
genotype/variation
Clonal FOXA2 doxycycline inducible cells
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Tagmentation was performed on whole nuclei at 37C for 45 minutes as previously described in (Buenrostro et al., 2013). DNA was isolated on PCR min-elute columns (Qiagen) and a small amount of the DNA was amplified for 9, 12 and 15 cycles to determine optimal cycling conditions. Nextera amplified