GSM2428951: ADU 0B9ZK input IP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
Neutrophils
MeSH Description
Immature neutrophils.
Attributes by original data submitter
Sample
source_name
active disease, treated
cell type
neutrophils
disease state
Juvenile Idiopathic Arthritis
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Peripheral blood mononuclear cells (PBMC) were separated from neutrophils and red blood cells by density-gradient centrifugation. Red cells were removed from neutrophils by hypotonic lysis. Neutrophils were crosslinked using 1% formaldehyde in 10 ml PBS for 10 min at room temperature. Crosslinking was quenched by adding 1× glycine. ChIP was performed following the protocol (Cell Signaling Technologies, Inc.). DNA-sequencing was conducted using the Illumina HiSeq 2500.