Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Cell line

Cell type

S2R+

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

ChIP Seq with RnpS1 HA tagged Cells

cell line

S2R+ Cells

cell type

Embryonic

chip antibody

anti-HA (F-7) (Santa Cruz, catalog# sc-7392, lot# I0916)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was recovered after reverse crosslinking and phenol chloroform extraction. After precipitating and pelleting, DNA was dissolved in 30 µl of TE After checking enrichment the recovered DNA was converted into libraries using NebNext Ultra DNA II library preperation kit, following manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

15102330

Reads aligned (%)

97.2

Duplicates removed (%)

14.7

Number of peaks

3185 (qval < 1E-05)

dm3

Number of total reads

15102330

Reads aligned (%)

97.6

Duplicates removed (%)

11.5

Number of peaks

3331 (qval < 1E-05)