Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BCL3

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

dev stage
adult
donor ID
ENCDO000AAZ
age
58 year
sex
male
lab
Tim Reddy, Duke
treatments
treatment_term_id: CHEBI:41879; treatment_term_name: dexamethasone; treatment_type: chemical; duration: 2; duration_units: hour;
sample type
immortalized cell line
cell line
A549

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
general protocol: https://www.encodeproject.org/documents/e21fb833-cd54-4c37-a3b6-440732909433/@@download/attachment/01_ChIP_DEX_Harvest_Reddy_GGR.pdf extraction protocol: https://www.encodeproject.org/documents/4ac7e9ce-4a92-42da-a1ab-299d4205eb96/@@download/attachment/02_ChIP_shearing_IP_reverse_v.2_Reddy_GGR.pdf treatment protocol: https://www.encodeproject.org/documents/1bca5eae-b9ce-4df0-b401-612924dc53a4/@@download/attachment/03_KAPA_Hyper_Prep_Kit_KR0961-TDSv2.15_final_24%20rxn_Reddy_GGR.pdf general protocol: https://www.encodeproject.org/documents/0d841e8a-a32d-416e-8f99-9db162bd09be/@@download/attachment/03_notes_Reddy_GGR.pdf

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
19170704
Reads aligned (%)
97.6
Duplicates removed (%)
2.1
Number of peaks
12585 (qval < 1E-05)

hg19

Number of total reads
19170704
Reads aligned (%)
97.1
Duplicates removed (%)
2.9
Number of peaks
12557 (qval < 1E-05)

Base call quality data from DBCLS SRA