Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
CD4 CD8 double positive cells
NA
NA

Attributes by original data submitter

Sample

source_name
adult pre-selection DP thymocyte
developmental stage
adult
genotype/variation
control
cell type
thymocyte

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Nuclei were prepared from 25,000-sorted cells. The transposase reactions were carried using Nextera DNA Library Preparation Kit (Illumina). Purified DNA fragments were amplified by PCR. The purified library was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
35478087
Reads aligned (%)
90.0
Duplicates removed (%)
51.6
Number of peaks
17447 (qval < 1E-05)

mm9

Number of total reads
35478087
Reads aligned (%)
89.8
Duplicates removed (%)
51.7
Number of peaks
17263 (qval < 1E-05)

Base call quality data from DBCLS SRA