Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Orc2

Cell type

Cell type Class
Larvae
Cell type
Fat Body
MeSH Description
A nutritional reservoir of fatty tissue found mainly in insects and amphibians.

Attributes by original data submitter

Sample

source_name
Male OrR 92hr AEL larval fat body tissue, ORC2 ChIP
strain/background
OregonR
genotype/variation
wild-type
Sex
male
tissue
fat body
developmental stage
92hr after egg laying (AEL) - Mid-3rd instar larvae
chip antibody
anti-ORC2 (Steve Bell lab, MIT)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fat body tissue was dounced homogenized and then sonicated using a Bioruptor 300 (Diagenode) prior to ChIP with anti-ORC2 antibody. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (Part # E7645S) according to manufacturer's recommendations with indexing primers for multiplexing.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
6841104
Reads aligned (%)
62.4
Duplicates removed (%)
40.2
Number of peaks
3469 (qval < 1E-05)

dm6

Number of total reads
6841104
Reads aligned (%)
62.4
Duplicates removed (%)
40.7
Number of peaks
2730 (qval < 1E-05)

Base call quality data from DBCLS SRA