Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
Prostate cancer-associated fibroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
Primary Fibroblast
cell type
Human prostate cancer-associated fibroblasts
passages
P5-P7
chip antibody
AR (Santa Cruz, H280, sc-13062, Lot D0414)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and AR-DNA complexes were isolated with antibody. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina’s NextSeq 500 (75 nt reads, single end)

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
37509144
Reads aligned (%)
80.0
Duplicates removed (%)
12.4
Number of peaks
1273 (qval < 1E-05)

hg38

Number of total reads
37509144
Reads aligned (%)
81.3
Duplicates removed (%)
11.4
Number of peaks
1399 (qval < 1E-05)

Base call quality data from DBCLS SRA