Antigen

Antigen Class

TFs and others

Antigen

Arid3a

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

ES cells

cell type

J1 embryonic stem cells

chip antibody

Arid3a

chip antibody vendor

1:1 mixed with home-made (Webb et al., 2011) and from Santa Cruz Biotechnology

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq, total RNAs were extracted using Rneasy plus mini kit (Qiagen). RNA- and ChIP-sequencing libraries were generated using library prep kits (NEB) according to the manufacturer’s instructions. ATAC-seq samples were performed as previously described (Buenrostro et al., 2015)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

32438630

Reads aligned (%)

78.3

Duplicates removed (%)

31.4

Number of peaks

798 (qval < 1E-05)

mm9

Number of total reads

32438630

Reads aligned (%)

78.2

Duplicates removed (%)

31.5

Number of peaks

730 (qval < 1E-05)