Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Naïve human embryonic stem cells

developmental stage

naive

cell type

embryonic stem cell

growth condition

GSKi + MEKi (2i), Lif, IGF1, FGF

chip antibody

Active Motif anti-H3K4me3, cat 39159

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq as outlined in Hawkins et al., 2013; DNase-HiC similar to Deng et al., 2015

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

15960227

Reads aligned (%)

90.9

Duplicates removed (%)

5.5

Number of peaks

14267 (qval < 1E-05)

hg19

Number of total reads

15960227

Reads aligned (%)

90.0

Duplicates removed (%)

7.2

Number of peaks

14411 (qval < 1E-05)