GSM2410318: SK-N-SH EBF3 WT FLAG Rep 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EBF3
Cell type
Cell type Class
Neural
Cell type
SK-N-SH
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
SK-N-SH, WT EBF3-FLAG, FLAG ChIP
cell line
SK-N-SH (ATCC HTB-11)
stable expression
WT EBF3-FLAG
chip antibody
anti-FLAG(M2) (Sigma-1804, Lot# SLBK1346V)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20x10e6 cells for two biological replicates for each vector were crosslinked in 1% formaldehyde, sonicated to fragment chromatin using the BioRuptor Twin Sonicator (Diagenode), and immunoprecipitated using monoclonal anti-FLAG M2 antibody (Sigma). Samples were reverse-crosslinked and recovered DNA was used as input for Illumina sequencing library preparation. ChIP-seq was performed as previously described (Reddy et al. 2009).