Curated Sample Data


Genome
hg19
Antigen Class
Histone
Antigen
H3K4me2
Cell type Class
Blood
Cell type
Lymphoid Progenitor Cells

Cell type information


MeSH Description
Stem cells from which B-LYMPHOCYTES; T-LYMPHOCYTES; NATURAL KILLER CELLS; and some DENDRITIC CELLS derive.

Attributes by Original Data Submitter


source_name
peripheral blood
tissue
peripheral blood
cell type
Innate lymphoid cell precursors (ILCPs)
antibody
anti-H3K4Me2, Abcam ab32356

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Human peripheral blood mononuclear cells (PBMC) from peripheral blood were isolated by Ficoll-Paque density gradient centrifugation. Cells were isolated by FACS (ILCP: viable CD45+ Lin- (CD3- CD4- CD5- TCRαβ- TCRγδ- CD14- CD19-) CD7+ CD127+ CRTh2- CD117+ cells, HSC: CD45+ CD34+) Nextera DNA library construction (as described in Schmidl et al. Nature Methods 2015).

Platform Information


instrument_model
NextSeq 500

External Database Query

Logs in read processing pipeline


Number of total reads
33177243
Reads aligned (%)
94.2
Duplicates removed (%)
8.7
Number of peaks
1918 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA