Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS_PolII_UV

cell line

U2OS

cell type

Human osteosarcoma cell line

treated with

UV light (40 J/m2, 1 hour recovery)

chip antibody

Pol II (N-20), Santa Cruz, Cat# sc-899

Sequenced DNA Library

library_name

ERROR: cannot retrieve data from NCBI: too many requests

library_strategy

ERROR: cannot retrieve data from NCBI: too many requests

library_source

ERROR: cannot retrieve data from NCBI: too many requests

library_selection

ERROR: cannot retrieve data from NCBI: too many requests

library_construction_protocol

ERROR: cannot retrieve data from NCBI: too many requests

Sequencing Platform

instrument_model

ERROR: cannot retrieve data from NCBI: too many requests

cycle_sequence

ERROR: cannot retrieve data from NCBI: too many requests

cycle_count

ERROR: cannot retrieve data from NCBI: too many requests

flow_sequence

ERROR: cannot retrieve data from NCBI: too many requests

flow_count

ERROR: cannot retrieve data from NCBI: too many requests

key_sequence

ERROR: cannot retrieve data from NCBI: too many requests

hg38

Number of total reads

30366168

Reads aligned (%)

78.4

Duplicates removed (%)

27.8

Number of peaks

1476 (qval < 1E-05)

hg19

Number of total reads

30366168

Reads aligned (%)

77.5

Duplicates removed (%)

29.5

Number of peaks

1464 (qval < 1E-05)