Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pancreas

Cell type

Islets of Langerhans

MeSH Description

Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Sample

source_name

Isolated islets

strain

C57BL/6J

tissue

islets

age

10 weeks

genotype

HDAC3betaKO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Islets were isolated and processed immdiately for RNA extraction with Trizol, or fixed with 1% formaldehyde for ChIP. Libraries were prepared using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

17593841

Reads aligned (%)

71.2

Duplicates removed (%)

26.6

Number of peaks

213 (qval < 1E-05)

mm9

Number of total reads

17593841

Reads aligned (%)

71.1

Duplicates removed (%)

26.7

Number of peaks

148 (qval < 1E-05)