Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

strain

C57B/6

tissue

liver

age

14 weeks old

gender

male

antibody

H3K27Ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Livers were isolated, flash frozen on liquid nitrogen, and later processed for RNA extraction with Trizol, or fixed with 1% formaldehyde for ChIP. Libraries were prepared using standard Illumnia protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

29459018

Reads aligned (%)

98.1

Duplicates removed (%)

17.8

Number of peaks

37660 (qval < 1E-05)

mm9

Number of total reads

29459018

Reads aligned (%)

98.0

Duplicates removed (%)

17.9

Number of peaks

37672 (qval < 1E-05)