GSM2401445: BJ ATAC 48h R2; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Epidermis
Cell type
BJ
Primary Tissue
Skin
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
ATACseq Post-FOXA2
cell line background
BJ-5ta
cell type
immortalized BJ foreskin fibroblast cell line
genotype/variation
FOXA2 doxycycline inducible cell line
treated with
0.5 ug/ml doxycycline
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Tagmentation was performed on whole nuclei at 37C for 45 minutes as previously described in (Buenrostro et al., 2013). DNA was isolated on PCR min-elute columns (Qiagen) and a small amount of the DNA was amplified for 9, 12 and 15 cycles to determine optimal cycling conditions. Nextera amplified