GSM2394711: TIG120 H3K36me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K36me3
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
Human dermal fibroblast
cell type
Human dermal fibroblast
antibody
Active Motif 61101 (Rabbit polyclonal anti-Histone H3 (tri-methyl K36))
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were subjected to cross-linking. After fixation with 1% formamide, cells were sonicated to shear DNA. DNA-protein complexes were incubated with Protein G beads (GE Healthcare) and anti-histone modification polyclonal antibodies (H3K4me1/H3K4me3/H3K27ac/H3K36me3). After washing the beads, immunoprecipitants were eluted with 30 µL of Tris buffer. Multiplexed ChIP-seq libraries were prepared from 10 ng of immunoprecipitated DNA fragments using NEBNext ChIP-seq Library Prep Master Mix (New England BioLabs). For sequencing using GAIIx (Illumina), cluster generation was performed using TruSeq SR Cluster Kit v2 (Illumina). Each lane of flow cells contained one sample. Sequencing was performed in single-read run mode with a total 76 cycles, including a 75-bp read and one cycle for phasing.