Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Blood

Cell type

Granulocytes

MeSH Description

Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.

Sample

source_name

Granulocytes

genotype

wildtype

cell markers

Mac1+Gr-1+

tissue

bone marrow

chip-antibody

ab8895, Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Genomic DNA extraction protocol not provided. Precipitated DNA were mixed with 0-2 ng E. Coli DNA, depending on the amount of ChIP DNA isolated. This mix was amplified using NEB Next ChIP-seq sample prep reagent set 1 (New England Biolabs) according to manufacturer’s protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

203554586

Reads aligned (%)

75.8

Duplicates removed (%)

40.9

Number of peaks

115688 (qval < 1E-05)

mm9

Number of total reads

203554586

Reads aligned (%)

75.7

Duplicates removed (%)

40.9

Number of peaks

115744 (qval < 1E-05)