GSM2384786: Pol II ChIPseq NPC; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Pluripotent stem cell
Cell type
mESC derived neural cells
NA
NA
Attributes by original data submitter
Sample
source_name
Cell line_PolII_ChIP_no treatment
cell line
V6.5
antibody
Anti-Pol II antibody, sc-56767
cell type
ESC-derived NPCs
treatment
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with 1% formaldehyde. Cell lysates were obtained from sonicated nuclei and protein-DNA complexes were isolated with indicated antibodies. Libraries were prepared with KAPA LTP kit according to manufacturer's protocol.