Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.
Attributes by Original Data Submitter
Hematopoeitic stem cells
8-12 weeks old
long-term hematopoietic stem cell
Metadata from Sequence Read Archive
10,000 HSPCs were sorted into PBS buffer and cross-linked with 1% formaldehyde at RT for 10 min, and 0.125 M glycine was added to stop the reaction at RT for 5 min, followed by washing once with ice-cold Hank's buffered salt solution (HBSS) containing protease inhibitor cock¬tail (PIC). Libraries were constructed with the KAPA library preparation kit for Illumina (Kapa Biosystems).