Curated Sample Data


Genome
mm9
Antigen Class
Input control
Antigen
Input control
Cell type Class
Blood
Cell type
Hematopoietic Stem Cells

Cell type information


MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by Original Data Submitter


source_name
Hematopoeitic stem cells
strain
C57BL/6
age
8-12 weeks old
cell type
long-term hematopoietic stem cell
chip antibody
None

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10,000 HSPCs were sorted into PBS buffer and cross-linked with 1% formaldehyde at RT for 10 min, and 0.125 M glycine was added to stop the reaction at RT for 5 min, followed by washing once with ice-cold Hank's buffered salt solution (HBSS) containing protease inhibitor cock¬tail (PIC). Libraries were constructed with the KAPA library preparation kit for Illumina (Kapa Biosystems).

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
39899161
Reads aligned (%)
97.9
Duplicates removed (%)
13.2
Number of peaks
575 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA