ChIP-Atlas: SRX2322341

Antigen

Cell type Class: Blood
Cell type: Plasma Cells
MeSH Description: Specialized forms of antibody-producing B-LYMPHOCYTES. They synthesize and secrete immunoglobulin. They are found only in lymphoid organs and at sites of immune responses and normally do not circulate in the blood or lymph. (Rosen et al., Dictionary of Immunology, 1989, p169 & Abbas et al., Cellular and Molecular Immunology, 2d ed, p20)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: Cells were pelleted at 500g for 10 min and lysed in nuclei lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL). Nuclei were pelleted at 500g for 30 min and tagmented with Tn5 (Illumina) for 60 min at 37°C prior to protein digestion with 2 mL 10 mg/mL Proteinase K at 40°C for 40 minutes. Libraries were size selected with SPRI beads (AMPure) prior to 12 rounds of PCR amplification.