PE-specific germinal center B cell (B220+ Fas+ GL7+ PE+), Dnmt3-deficient
background/strain
B6;129S4-Dnmt3atm3.1Enl Dnmt3btm5.1Enl/Mmnc
genotype/variation
Cd19cre/wtDnmt3afl/flDnmt3bfl/fl
gender
male
tissue
lymph node
cell type
PE-specific germinal center B cell (B220+ Fas+ GL7+ PE+)
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were pelleted at 500g for 10 min and lysed in nuclei lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL). Nuclei were pelleted at 500g for 30 min and tagmented with Tn5 (Illumina) for 60 min at 37°C prior to protein digestion with 2 mL 10 mg/mL Proteinase K at 40°C for 40 minutes. Libraries were size selected with SPRI beads (AMPure) prior to 12 rounds of PCR amplification.