Antigen

Antigen Class

TFs and others

Antigen

VDR

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

human monocytic cell line THP-1

cell line

monocytic cell line THP-1

treatment

100 nM 1,25(OH)2D3

time

2 h

chip antibody

VDR (Cell Signaling Technologies CST 12550)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

proteins were crosslinked with formaldehyde to DNA, then VDR-bound fragments were pulled down by chromatin immunoprecipitation ChIP-seq library was prepared from 5-10 ng ChIP DNA with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) per manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

10602580

Reads aligned (%)

99.1

Duplicates removed (%)

2.3

Number of peaks

506 (qval < 1E-05)

hg19

Number of total reads

10602580

Reads aligned (%)

98.2

Duplicates removed (%)

3.6

Number of peaks

659 (qval < 1E-05)