Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SUPT5H

Cell type

Cell type Class
Blood
Cell type
MOLT-4
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
T-ALL
cell line
MOLT4
cell type
T-acute lymphoblastic leukemia (T-ALL) cell line
treated with
NVP-2 (250 nM) for 2 hours
chip antibody
SPT5
chip antibody vendor
Santa Cruz
input sample
MOLT4_input
sequencing barcode
TGTGGTTG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Standard DNA extraction from MOLT4 cell lines using cell lysis. Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 40% amplitude) on ice library prep with Rubicon ThruPlex kit

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
66172490
Reads aligned (%)
62.9
Duplicates removed (%)
23.2
Number of peaks
17055 (qval < 1E-05)

hg38

Number of total reads
66172490
Reads aligned (%)
63.8
Duplicates removed (%)
22.7
Number of peaks
17689 (qval < 1E-05)

Base call quality data from DBCLS SRA