Antigen

Antigen Class

TFs and others

Antigen

SUPT5H

Cell type

Cell type Class

Blood

Cell type

MOLT-4

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

T-ALL

cell line

MOLT4

cell type

T-acute lymphoblastic leukemia (T-ALL) cell line

treated with

NVP-2 (250 nM) for 2 hours

chip antibody

SPT5

chip antibody vendor

Santa Cruz

input sample

MOLT4_input

sequencing barcode

TGTGGTTG

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Standard DNA extraction from MOLT4 cell lines using cell lysis. Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 40% amplitude) on ice library prep with Rubicon ThruPlex kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

66172490

Reads aligned (%)

82.5

Duplicates removed (%)

22.7

Number of peaks

17689 (qval < 1E-05)

hg19

Number of total reads

66172490

Reads aligned (%)

81.8

Duplicates removed (%)

23.2

Number of peaks

17055 (qval < 1E-05)