Wild-type MEFs immortalized with SV40 large T antigen and treated with MG132.
cell type
Mouse enbryonic fibroblasts
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated using antibody to Nrf1 and Dynabeads-proteinA and -proteinG Libraries were prepared according to NuGEN's instructions accompanying the Ovation Ultralow DR Multiplex System 1-8 (NuGEN, 0330-32).