Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse ESCs and mouse extended PSCs, input
cell line
mc2i, mc6-1
cell type
Mouse embryonic stem cells and mouse extended pluripotent stem cells
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq library was prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
37123558
Reads aligned (%)
97.3
Duplicates removed (%)
6.1
Number of peaks
251 (qval < 1E-05)

mm9

Number of total reads
37123558
Reads aligned (%)
97.2
Duplicates removed (%)
6.2
Number of peaks
220 (qval < 1E-05)

Base call quality data from DBCLS SRA