Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Mouse ESCs, H3K27me3 ChIP

cell line

mc2i

cell type

Mouse embryonic stem cells

chip antibody

anti-H3K27me3 (Millipore, cat. no. 07-449, lot# 2194165)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-Seq library was prepared using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

35303957

Reads aligned (%)

98.4

Duplicates removed (%)

6.3

Number of peaks

278 (qval < 1E-05)

mm9

Number of total reads

35303957

Reads aligned (%)

98.3

Duplicates removed (%)

6.4

Number of peaks

234 (qval < 1E-05)