Antigen

Antigen Class

Histone

Antigen

H4K5ac

Cell type

Cell type Class

Liver

Cell type

HuH-7

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HuH7 cells

treatment

10ng/ml IL-1alpha for 1 h

antibody

H4K5ac (Millipore, 17-10045)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP lysis buffer (1% SDS, 10mM EDTA, 50mM Tris pH 8.1, 1mM PMSF, Roche protease inhibitor mix), followed by IP and purification of DNA Sequencing libraries were prepared from 10 ng of immunoprecipitated DNA with the Illumina ChIPSeq DNA Sample Prep Kit according to Illuminas’ instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31980162

Reads aligned (%)

98.7

Duplicates removed (%)

34.7

Number of peaks

21597 (qval < 1E-05)

hg19

Number of total reads

31980162

Reads aligned (%)

98.3

Duplicates removed (%)

35.3

Number of peaks

21605 (qval < 1E-05)