Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mESC
cell type
mESC
genotype
WT
treatment
None
chip antibody
H3K4me1 (39297, Active Motif)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibodies againts the spcific proteins of interest in each case. DNA libraries were prepared using the Illumina TruSeq RNA sample preparation Kit v2 starting from the end repair step of the protocol. Up to 100 ng ChIP DNA was used as starting material. After end repair and A-tailing, indexing adapters were ligated. The products were then purified and amplified for 18 PCR cycles to create the final libraries.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
24758866
Reads aligned (%)
96.1
Duplicates removed (%)
8.9
Number of peaks
1183 (qval < 1E-05)

mm9

Number of total reads
24758866
Reads aligned (%)
96.1
Duplicates removed (%)
8.9
Number of peaks
1146 (qval < 1E-05)

Base call quality data from DBCLS SRA