Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

reprogramming cells

reprogramming stage

6d

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

10139682

Reads aligned (%)

32.0

Duplicates removed (%)

1.0

Number of peaks

10940 (qval < 1E-05)

hg19

Number of total reads

10139682

Reads aligned (%)

31.8

Duplicates removed (%)

1.0

Number of peaks

10893 (qval < 1E-05)