Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

reprogramming cells

reprogramming stage

6d

chip antibody

H3K4me2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA DNA Library Preparation Kits.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

11322143

Reads aligned (%)

43.5

Duplicates removed (%)

2.6

Number of peaks

327 (qval < 1E-05)

hg19

Number of total reads

11322143

Reads aligned (%)

43.0

Duplicates removed (%)

2.7

Number of peaks

265 (qval < 1E-05)