Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Neural

Cell type

Hippocampus

MeSH Description

A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Sample

source_name

whole hippocampus

age

P30

tissue

brain

strain

C57B1/6J

antibody

k27ac

condition

Ehmt2 wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chipping for H3k27ac (Diagenode A5051-001P) was performed as in http://www.blueprint-epigenome.eu/UserFiles/file/Protocols/Histone_ChIP_July2014.pdf Library was synthetized with KAPA Hyper prep kit (KK8504) according to manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

47342231

Reads aligned (%)

96.1

Duplicates removed (%)

12.8

Number of peaks

40360 (qval < 1E-05)

mm9

Number of total reads

47342231

Reads aligned (%)

96.1

Duplicates removed (%)

12.9

Number of peaks

40370 (qval < 1E-05)