Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Neural

Cell type

Olfactory Bulb

MeSH Description

Ovoid body resting on the CRIBRIFORM PLATE of the ethmoid bone where the OLFACTORY NERVE terminates. The olfactory bulb contains several types of nerve cells including the mitral cells, on whose DENDRITES the olfactory nerve synapses, forming the olfactory glomeruli. The accessory olfactory bulb, which receives the projection from the VOMERONASAL ORGAN via the vomeronasal nerve, is also included here.

Sample

source_name

whole olfactory bulb

age

P30

tissue

brain

strain

C57B1/6J

antibody

k9me3

condition

Ehmt1 wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chipping for H3k9me3 (Diagenode A1671-001P) was performed as in http://www.blueprint-epigenome.eu/UserFiles/file/Protocols/Histone_ChIP_July2014.pdf Library was synthetized with KAPA Hyper prep kit (KK8504) according to manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

46165731

Reads aligned (%)

95.4

Duplicates removed (%)

37.9

Number of peaks

714 (qval < 1E-05)

mm9

Number of total reads

46165731

Reads aligned (%)

94.5

Duplicates removed (%)

37.6

Number of peaks

928 (qval < 1E-05)